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From Mice and Cows and Kangaroos to Dairy Industry Value

    White mouse sniffing at a glass flask

    Written by: Peter Williamson, Ph.D. | Issue # 12 | 2013

    • The complexities of lactation performance may be unraveled by genomics.
    • Genomics gives traditional genetics much greater resolution and accuracy.
    • Functional genomics deals with how genes make physiological sense.
    • Integration of the two adds much greater power to understanding lactation.
    • There are potential benefits across the dairy value chain from using these approaches.

    With the development of genomic tools for dairy cows, what value do studies of lactation genomics in mice and other animals hold for dairy innovation? A recent study reported in the January 2013 issue of Physiological Genomics (1) is the latest in a series of studies of lactation in mice that have involved scientists affiliated with the IMGC (2-7). This latest study focused on lactation performance, a correlate of milk output.

    The capacity of dairy cows to produce milk varies between cattle breeds and, to a lesser degree, within a breed. Geneticists have been studying and analyzing this variation for many decades and have developed selection procedures to capture the desired traits in more members of dairy herds. This has delivered considerable on-farm productivity increases for milk supply, but it relied on estimating the breeding value of a bull from a lengthy and costly system coupled with widespread herd recording for the important production characteristics or traits.

    The genomics era has now introduced DNA-based methods that provide greater accuracy in genotyping and the capability for more rapid selection of desirable traits using molecular breeding values (8). These developments also provide a way to incorporate more complexity in measurements that reflect characteristics that affect the physiology of dairy cows. The key to capitalizing on the power of this technology is to have the appropriate measurements, which can take considerable time when measuring multiple generations of dairy cow lactation performance. This is where laboratory studies with mice can contribute.

    So why choose mice? Although not identical, the physiology of the mammary gland is similar between species. The study published last month confirms that there are core physiological events that are reflected in the gene expression patterns of mice, and these are similar to cattle and other species during the lactation cycle. The advantage of studying mice is that they can produce multiple litters in a short space of time and hence have multiple lactations to study. Therefore, we also have the capacity to make lots of relevant measurements for analysis (7). This can help to develop selection procedures that have positive effects on dairy cow health and economic traits yet avoid unwanted deleterious effects, like reduced fertility.

    The genetic makeup of an animal can account for variation in traits in two ways: by affecting the structure of a functional protein or by affecting the amount of a protein that is produced. Structural variants that have a major effect have been identified in dairy cows, e.g., in the protein DGAT1 which influences milk fat. The impact of changing levels of gene expression, and thereby the proteins that they produce, is more subtle and is usually the result of multiple changes. It is also influenced by other events, like nutrition and energy use. We can measure these using genomic tools that capture a snapshot of how much a gene is turned on at any one time or place in the body.

    There may be thousands of changes occurring from one stage to another, and there is a high level of interconnectivity between the events. However, the events are not random; they are controlled in a coordinated manner. One way to think of it is that they group into patterns, and the change in patterns occurs in waves. An example of how these patterns emerge and change during lactation in mice is shown in the published paper. Interpretation of these patterns by comparing groups of studies like these is emerging, and the results may be assembled into biological networks. Ultimately, this will capture the most robust patterns that explain the complexity of the lactation system, and it can be reduced to key components. The advantage of having simple key measures of a complex system is that it will allow the development of real-time measures that can be incorporated into improving efficient dairy herd management systems.

    Consumer health and well-being are key drivers of product innovation for dairy. Sophisticated and real-time measures of milk production could capture valued milk components or assist in split herd management for specified milk composition. Mice can be used as a model here, too. Mouse milk composition varies with the type or strain of mouse, and the way in which it is managed. The impact of this diversity on growth and development characteristics of baby mice can be correlated and followed for the lifetime of the animals.

    So, what about the kangaroo? They represent both an extreme example of diversity in milk production and lactation biology, and they have the capacity to demonstrate how varied milk proteins can protect the newborn and affect developing tissues (9-11). Along with other species, they demonstrate the potential of milk, and they shed light on what may be possible with dairy. They also help to define those essential biological elements of any lactation system and so contribute to the development of a simplified system for applications in dairy cow management.

    Taken together, the use of mouse laboratory studies and the exploration of diversity in lactation between species will provide a valuable resource for dairy innovation into the future.

    References

    1. Wei J, Ramanathan P, Martin IC, Moran C, Taylor RM, Williamson P. (2013) Identification of gene sets and pathways associated with lactation performance in mice. Physiol Genomics: doi:10.1152/physiolgenomics.00139.2011.2. Anderson S, Rudolph MC, McManaman JL, Neville, MC. (2007) Key stages in mammary gland development. Secretory activation in the mammary gland: it’s not just about milk protein synthesis. Breast Cancer Res 9:204.

    3. Rudolph M, McManaman JL, Phang T, Russell T, Kominsky DJ, Serkova N, Stein T et al. (2007) Metabolic regulation in the lactating mammary gland: A lipid synthesizing machine. Physiol Genomics 28:323-336.

    4. Lemay DG, Neville MC, Rudolph MC, Pollard KS, German JB. (2007) Gene regulatory networks in lactation: identification of global principles using bioinformatics. BMC Syst Biol 1:56.

    5. Ramanathan P, Martin IC, Gardiner-Garden M, Thomson PC, Taylor RM, Ormandy CJ, Moran C, Williamson P. (2008) Transcriptome analysis identifies pathways associated with enhanced maternal performance in QSi5 mice. BMC Genomics 9:197.

    6. Rijnkels M, Freeman-Zadrowski C, Hernandez J, Potluri V, Wang L, Li W, Lemay DG. (2013) Epigenetic modifications unlock the milk protein gene loci during mouse mammary gland development and differentiation. PLoS One 8: e53270. doi: 10.1371/journal.pone.0053270.

    7. Hadsell DL, Wei J, Olea W, Hadsell LA, Renwick A, Thomson PC, Shariflou M, Williamson P. (2012) In silico QTL mapping of maternal nurturing ability with the mouse diversity panel. Physiol Genomics 44:787-798

    8. Moser G, Tier B, Crump RE, Khatkar MS, Raadsma HW. (2009) A comparison of five methods to predict genomics breeding values of dairy bulls from genome-wide SNP markers. Genet Sel Evol 41:56.

    9. Lefevre C, Sharp J, Nicholas K. (2010) Evolution of lactation: ancient origins and extreme adaptations of the lactation system. Annu Rev Genomics Human Genet 11:219-238.

    10. Daly KA, Digby MR, Lefevre C, Nicholas KR, Deane EM, Williamson P. (2008) Identification, characterization and expression of cathelicidin in the pouch young of tammar wallaby (Macropus eugenii). Comp Biochem Physiol B Biochem Mol Biol 149:524-533.

    11. Daly KA, Digby M, Lefevre C, Mailer S, Thomson P, Nicholas K, Williamson P. (2007) Analysis of the expression of immunoglobulins throughout lactation suggests two periods of immune transfer in the tammar wallaby (Macropus eugenii). Vet Immunol Immunopathol 120:187-200.

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